Arnold's seminar November 4, 2003 Oleg Karpenkov. On bi-cubic characterization polynomials for classes of 3x3 matrices with integer coefficients and distinct irrational eigenvalues. Abstract: We recall the definition of the bi-quadratic characterization polynomials for classes of 2x2 matrices and give the generalization to the three dimensional case (Q_A(x,y,z;m,n)). We show that every bi-cubic characterization polynomial is the product of two polynomials of degree 3 with integer coefficients It appears that one of these two polynomials is uniquely defined by any matrix of the class whereas the second one depends on the choice of the basis for the class. There exist some class of Frobenius matrices conjugate to the given class A iff there exist integer numbers (x,y,z;m,n) such that Q_A(x,y,z;m,n)=1 or Q_A(x,y,z;m,n)=-1. As a consequence we present the class of 3x3 matrices with integer coefficients and distinct irrational eigenvectors that is not conjugate to any class containing Frobenius matrices.